White spot syndrome virus WSSV Nucleic Acid Detection Kits
(PCR-Fluorescent Probe Method)
Intended use:
This kit is suitable for the detection of Shrimp white spot syndrome virus (WSSV) in shrimp samples according to the different size or infection stage of different tissue samples, used for the auxiliary diagnosis of shrimp white spot syndrome virus (WSSV) infection.
Principle:
This kit uses a specific fragment of white spot syndrome virus (WSSV) to design a primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclization activity of Taq enzyme cuts the 5 '-terminal fluoromeric group from the probe, making it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of white spot syndrome virus (WSSV) was detected in the completely closed reaction system.
Biokey Aquaculture PCR testing solution.pdf
Main components:
| Component | Specification |
| PCR reaction liquid | 24 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be interchangeable.
Sample requirements:
1. Sample type
Tissue samples from prawn.
2. Transport
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3.Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
4. Sample extraction&purification processing with professional machine below:
Using Micgene 242/244/244IVD and ABI QuantStudio5:
procedures are set as below:
| Steps | Cycle number | Temperature | Time |
| 1 | 1 | 95℃ | 3min |
| 2 | 45 | 95℃ | 10s |
| 60℃ | 30sCollect the fluorescent |
FAM was selected for detection channel.
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤45 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
procedures are set as below:
| Steps | Cycle number | Temperature | Time |
| 1 | 1 | 95℃ | 3min |
| 2 | 10 | 95℃ | 10s |
| 60℃ | 30s |
| 3 | 35 | 95℃ | 10s |
| 60℃ | 30sCollect the fluorescent |
FAM was selected for detection channel.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤35 |
| Negative | No Ct value or Ct value≥35 |
| Gray area | 30＜Ct<35 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 35 is considered as positive, and no Ct value or Ct value ≥35 is considered as negative.
Details please check from IFU!
We offer the full PCR testing solution to support the shrimp disease detection and monitoring on site.
Product Tags:
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